It's time for a leap forward in digital PCR technology. The Roche Digital LightCycler® System is the digital PCR instrument of tomorrow. With a unique combination of 3 nanowell plate configurations, 6 advanced optical channels, and 5x concentrated DNA and RNA master mixes, it has the potential to help your lab to make the leap from publishing research to producing clinically viable assays.
Digital LightCycler® System
Unleash the true power of digital PCR
IVD
For in vitro diagnostic use.
Make the leap from idea to impact
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The 4 pillars of advancement*
Sensitivity
The Digital LightCycler® System has an enhanced ability to detect minimal DNA or RNA molecules:
- High-sensitivity 20,000 partition plate w/ 45μl reaction volume
- 5x concentrated master mixes (RNA, DNA) enable high sample utilization, which means more confidence in results
- Little rain and clear cluster separation means setting thresholds is easy
Precision
The Digital LightCycler® System’s high resolution 100,000 plate offers laboratories:
- The ability to more likely discriminate small differences between samples
- Superior cluster tightness and clarity of results
- Absolute quantification of results
Flexibility
The Digital LightCycler® System brings all these advancements together on one system, offering a bold step forward in digital PCR:
- Choose batch size increments of between 8 and 96 samples per run
- Combine lanes for results of a single sample to increase resolution
- Simplify workflow with sample tracking and automated data analysis
- Enable high multiplexing with a 6 optical channel analyzer (Demonstrated with both DNA and RNA reagents)
- Address multiple challenges at once with three plates for different application needs (Universal, High Sensitivity, High Resolution)
Quick, clear, reproducible results give great confidence with the potential for shorter turnaround for publishable scientific research and the potential development of clinically viable assays.
Integration
Bringing it all together, the Digital LightCycler® System brings reliable confidence in results:
- Chemistry of generic reagents:
- 5x concentrated master mixes means greater potential sample volume input
- DNA and RNA optimized for multiplexing
- Filling dye control that validates partitioning
- 6 optical channels
- Nanowell plate types: Solid phase partitioning instead of droplets increases reliability and replicability
- Closed system minimizes the chance of amplicon contamination
- Laboratory Information Digital LightCycler® System connectivity means simple digital workflow and sample tracking
- Algorithm for sophisticated rejection of non-PCR artifacts
- Absolute quantification without the need for standard curves
Digital LightCycler® System Video
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Bespoke testing with 3 one-of-a-kind1-4 nanowell plates
The unique hexagonal-shaped nanowell partitions and the filling dye that validates partitioning gives the Digital LightCycler® System the ability to handle a wide variety of extracted samples such as FFPET, cfDNA, Whole Blood, and more.
Through these partitions, target sequences are efficiently concentrated within isolated microreactors. This concentration effect reduces template competition, thereby enabling the detection of rare mutations and allowing for a higher tolerance to inhibitors present in a sample.
High Resolution
15μL, 100,000 partitions
Copy Number Variation
NIPT
Human Genetic Disease
Universal Plate
30μL, 28,000 partitions
Gene Expression
Transplant Rejection
High Sensitivity
45μL, 20,000 partitions
Cell-free DNA
Oncology
Rare Mutation Detection
Increased flexibility with 6-channel multiplexing*
6 advanced optical channels enable a high degree of multiplexing for DNA or RNA targets.
- Batch size increments of between 8 and 96 samples per run
- Demonstrated with both DNA and RNA reagents
- Analyzer run times vary for different batch sizes to save time on smaller batches
High sample utilization with 5x concentrated DNA and RNA master mixes*
Get the most out of your samples with the Digital LightCycler® System's 5x concentrated master mixes.
- Improved sample percentage in each reaction means confidence in results
- 1 Step RNA (RT) with a hold time of just 15 minutes
- DNA and RNA optimized for multiplexing
- Filling dye control that validates partitioning
- Single tube
- 50-75% less primer/probe usage
Simple workflow and data tracking
1. First batches are created either manually or with orders from the Laboratory Information System (LIS). The system automatically sets up the layout and creates the samples.
2. The newly extracted samples are then transferred to the PCR where a pipette is used to mix the samples and the master mix and the nanowell plate of choice is scanned into the web application for full sample tracking.
3. The PCR mix is then transferred into the nanowell plate which is then loaded into the partitioning engine. This is done for each plate.
4. All the plates are then loaded into the Analyzer.
5. After the run is complete, positives and negatives are counted up and the Poisson calculation is applied.
6. Results are validated and then sent back to the LIS.
Focus on core tasks with comprehensive customer support
The Digital LightCycler® Service Software will help automate workflow and manage data ensuring no external calculations, software and processors will be required to collate, analyze, and contextualize assay results.
- Remote accessible software
- Automatic clustering
- Flexible for development of assays
- Analysis types
- Absolute Quantification
- Indel
- Copy Number Variation
- Full sample tracking
- Laboratory Information System interface
Designed to make workflows simple and easy
Find out how the latest digital PCR systems are impacting modern research laboratories and opening up new avenues for discovery.
A comprehensive suite of powerful features
View Full TableA comprehensive suite of powerful features
Backed up by a strong history of IVD leadership and recognition with the original LightCycler® System, the Digital LightCycler® has a combination of unique features that make it a powerful competitor in the digital PCR space.1-4
Backed up by a strong history of IVD leadership and recognition with the original LightCycler® System, the Digital LightCycler® has a combination of unique features that make it a powerful competitor in the digital PCR space.1-4
Competitor A |
Competitor B |
Competitor C |
Competitor D |
Roche |
|
Partitions |
20,000 | 8,500 - 26,000 | 20,000 - 30,000 | 20,000 | 20,000 - 28,000 - 100,000 |
Samples |
1 - 96 | 24, 96 | 4,12 | 1-16 | 8-96 |
Concentration on MMx |
2x, 4x | 4x | 5x, 10x | 2x | 5x |
Channels |
4, 6 | 2, 5 | 6 | 4 | 6 |
Total volume |
20μL | 12μL, 40μL | 7μL, 25μL | 20μL | 15μL - 45μL |
MD Label |
Yes | No | No | No | Yes |
Unambiguous thresholding = Confident results
Backed up by a strong history of IVD leadership and recognition with the original LightCycler® System, the Digital LightCycler® has a combination of unique features that make it a powerful competitor in the digital PCR space1-4.
- Clear cluster separation of positives and negatives
- Very little rain
Roche Digital LightCycler® System
Competitor A
Competitor B
A wide range of research possibilities
View Full TableA wide range of research possibilities
The Digital LightCycler® System’s unique precision and sensitivity capabilities make it ideally suited for a number of real-world applications and research areas:
The Digital LightCycler® System’s unique precision and sensitivity capabilities make it ideally suited for a number of real-world applications and research areas:
| Application | Oncology | Rare species and variant detection |
Microsatellite instability detection |
Pathogen load |
Digital LightCycler® System Potential |
Known specific variants of interest and tracking of treatment efficacy and disease progression | Absolute quantification without a standard curve | Hyper sensitivity | Absolute quantification without a standard curve |
| Application | Graft rejection monitoring in transplant cases | ID - Environmental surveillance |
Pharma - Large clinical trials |
Noninvasive prenatal testing |
Digital LightCycler® System Potential |
Ability to measure graft cfDNA (est. Molecular Market 600 mio CHF) | High sensitivity and precision. Inhibitors for particular sample types may mask NA content making difficult the rtPCR curve analysis |
Large volume testing for specific biomarkers & larger accuracy than rtPCR |
Limited accuracy with antigen testing |
Registration status
IVD
Making the leap from idea to impact
Modern clinical research demands a leap forward in instrumentation capability. With its flexibility, quality and advances in sensitivity and precision, the Digital LightCycler® System can help laboratories push the boundaries of clinical research forward
Unleash the true power of digital PCR with the Roche Digital LightCycler® System.
* Roche data on file: DH_02365.01_031B_Digital_LightCycler_Reagent_Feasibility_Report_v3, Document Number: 0000000000001200000501942
Roche data on file: DH-02365.01-500E_Digital LightCycler System Performance report
Roche data on file: DHF Digital_LightCycler_Reagent_Feasibility_Report
Roche data on file: DH-02365.01-008B_Digital_LightCycler_System_Feasibility_Report
- https://www.qiagen.com/us/products/instruments-and-automation/pcr-instruments/qiacuity-digital-pcr-system/ (last accessed on 08/20/2022)
- https://www.bio-rad.com/en-us/life-science/digital-pcr/qx-one-droplet-digital-pcr-ddpcr-system (last accessed on 08/20/2022)
- https://www.stillatechnologies.com/6-color-dpcr/ (last accessed on 08/20/2022)
- https://www.thermofisher.com/order/catalog/product/4489084 (last accessed on 08/20/2022)
